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Grace Bio-Labs custom nitrocellulose coated microarray slides
Mean <t>microarray</t> antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.
Custom Nitrocellulose Coated Microarray Slides, supplied by Grace Bio-Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+slide/coated+nitrocellulose+slides/pmc12977535-119-8-12
Average 86 stars, based on 1 article reviews
custom nitrocellulose coated microarray slides - by Bioz Stars, 2026-10
86/100 stars

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1) Product Images from "Proteome microarray-guided identification of mycobacterial antigens and ELISA-based peptide mapping for improved serological detection of Mycobacterium bovis infection in European badgers"

Article Title: Proteome microarray-guided identification of mycobacterial antigens and ELISA-based peptide mapping for improved serological detection of Mycobacterium bovis infection in European badgers

Journal: Journal of Clinical Microbiology

doi: 10.1128/jcm.01260-25

Mean microarray antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.
Figure Legend Snippet: Mean microarray antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.

Techniques Used: Microarray, Infection, Comparison

Mean microarray antigen spot intensities from TB-infected and BCG-vaccinated badger serum samples. Gray bars represent sera from TB-infected badgers collected 12–13 weeks after experimental infection; white bars represent sera from badgers that received ( a ) a single BCG vaccination or ( b ) two BCG vaccinations. Mean spot intensities (log₂-normalized) are shown with 95% confidence interval error bars. Independent-samples t -tests were used to compare antigen intensities between TB-infected and BCG-vaccinated groups. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown; ***: P < 0.001.
Figure Legend Snippet: Mean microarray antigen spot intensities from TB-infected and BCG-vaccinated badger serum samples. Gray bars represent sera from TB-infected badgers collected 12–13 weeks after experimental infection; white bars represent sera from badgers that received ( a ) a single BCG vaccination or ( b ) two BCG vaccinations. Mean spot intensities (log₂-normalized) are shown with 95% confidence interval error bars. Independent-samples t -tests were used to compare antigen intensities between TB-infected and BCG-vaccinated groups. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown; ***: P < 0.001.

Techniques Used: Microarray, Infection, Comparison

Related Articles

Saline:

Article Title: Spatial omics-based machine learning algorithms for the early detection of hepatocellular carcinoma
Article Snippet: Goat anti-Human IgG (#A80-104A) and Goat anti-Human Transferrin (#A80-128A) antibodies were obtained from Bethyl Laboratories (Montgomery, TX). .. The antibodies were first diluted in Phosphate Buffered Saline 1× solution (PBS 10×, BP39920 from Fisher Scientific) and HPLC grade water (W5-4 from Fisher Scientific) at a final concentration of 40 ng/μl and using a Picus Electronic Single Channel Pipette from Sartorius (Goettingen, Germany), 1 μl of each antibody was spotted on Nitrocellulose slides (SKU470639 from Grace Bio-Labs) on the top of which were clipped ProPlate 8 well slide modules (Path Protein Microarray Slides, 25 × 25 × 1 mm, #805025 from Grace Bio-Labs, Bend, OR). ..

High Performance Liquid Chromatography:

Article Title: Spatial omics-based machine learning algorithms for the early detection of hepatocellular carcinoma
Article Snippet: Goat anti-Human IgG (#A80-104A) and Goat anti-Human Transferrin (#A80-128A) antibodies were obtained from Bethyl Laboratories (Montgomery, TX). .. The antibodies were first diluted in Phosphate Buffered Saline 1× solution (PBS 10×, BP39920 from Fisher Scientific) and HPLC grade water (W5-4 from Fisher Scientific) at a final concentration of 40 ng/μl and using a Picus Electronic Single Channel Pipette from Sartorius (Goettingen, Germany), 1 μl of each antibody was spotted on Nitrocellulose slides (SKU470639 from Grace Bio-Labs) on the top of which were clipped ProPlate 8 well slide modules (Path Protein Microarray Slides, 25 × 25 × 1 mm, #805025 from Grace Bio-Labs, Bend, OR). ..

Concentration Assay:

Article Title: Spatial omics-based machine learning algorithms for the early detection of hepatocellular carcinoma
Article Snippet: Goat anti-Human IgG (#A80-104A) and Goat anti-Human Transferrin (#A80-128A) antibodies were obtained from Bethyl Laboratories (Montgomery, TX). .. The antibodies were first diluted in Phosphate Buffered Saline 1× solution (PBS 10×, BP39920 from Fisher Scientific) and HPLC grade water (W5-4 from Fisher Scientific) at a final concentration of 40 ng/μl and using a Picus Electronic Single Channel Pipette from Sartorius (Goettingen, Germany), 1 μl of each antibody was spotted on Nitrocellulose slides (SKU470639 from Grace Bio-Labs) on the top of which were clipped ProPlate 8 well slide modules (Path Protein Microarray Slides, 25 × 25 × 1 mm, #805025 from Grace Bio-Labs, Bend, OR). ..

Transferring:

Article Title: Spatial omics-based machine learning algorithms for the early detection of hepatocellular carcinoma
Article Snippet: Goat anti-Human IgG (#A80-104A) and Goat anti-Human Transferrin (#A80-128A) antibodies were obtained from Bethyl Laboratories (Montgomery, TX). .. The antibodies were first diluted in Phosphate Buffered Saline 1× solution (PBS 10×, BP39920 from Fisher Scientific) and HPLC grade water (W5-4 from Fisher Scientific) at a final concentration of 40 ng/μl and using a Picus Electronic Single Channel Pipette from Sartorius (Goettingen, Germany), 1 μl of each antibody was spotted on Nitrocellulose slides (SKU470639 from Grace Bio-Labs) on the top of which were clipped ProPlate 8 well slide modules (Path Protein Microarray Slides, 25 × 25 × 1 mm, #805025 from Grace Bio-Labs, Bend, OR). ..

Microarray:

Article Title: Spatial omics-based machine learning algorithms for the early detection of hepatocellular carcinoma
Article Snippet: Goat anti-Human IgG (#A80-104A) and Goat anti-Human Transferrin (#A80-128A) antibodies were obtained from Bethyl Laboratories (Montgomery, TX). .. The antibodies were first diluted in Phosphate Buffered Saline 1× solution (PBS 10×, BP39920 from Fisher Scientific) and HPLC grade water (W5-4 from Fisher Scientific) at a final concentration of 40 ng/μl and using a Picus Electronic Single Channel Pipette from Sartorius (Goettingen, Germany), 1 μl of each antibody was spotted on Nitrocellulose slides (SKU470639 from Grace Bio-Labs) on the top of which were clipped ProPlate 8 well slide modules (Path Protein Microarray Slides, 25 × 25 × 1 mm, #805025 from Grace Bio-Labs, Bend, OR). ..

Article Title: Triple-serotype chimeric oncolytic adenovirus exerts multiple synergistic mechanisms against solid tumors
Article Snippet: .. On-slide tissue permeabilization, cDNA synthesis and sequencing: After brightfield imaging, the ST slide was prewarmed to 42 °C and attached to a microarray slide module (Grace Biolabs). i. Permeabilization Enzyme (70 μl) was added to B1 along the side of the well (30 min) and tap cassette; ii. ..

Article Title: A Novel Mass Spectrometry Platform for Multiplexed N-glycoprotein Biomarker Discovery from Patient Biofluids by Antibody Panel Based N-glycan Imaging
Article Snippet: Slides were imaged on a solariX Legacy 7T FT-ICR mass spectrometer (Bruker) equipped with a matrix-assisted laser desorption/ionization (MALDI) source. .. Nitrocellulose-coated glass microscope slides (PATH microarray slides) and well slide modules (ProPlate Multi-Array Slide System, 24-well) were obtained from Grace Bio-Labs (Bend, OR). .. Trifluoroacetic acid, α-cyano-4-hydroxycinnamic acid, octyl-β-D-glucopyranoside, human alpha-1-antitrypsin, and stock human serum were obtained from Sigma Aldrich (St. Louis, MO).

Article Title: SARS-CoV-2 Antibody Signatures for Predicting the Outcome of COVID-19
Article Snippet: .. 150 151 Protein microarray fabrication 152 The microarray used for serum IgM and IgG profiling was prepared as described previously.17, 23 20 153 proteins of SARS-CoV-2 with indicated concentrations, along with the negative (GST, Biotin-154 control, and eGFP) and positive controls (Human IgG, Human IgM, and ACE2-Fc), were printed in 155 quadruplicate on PATH substrate slide (Grace Bio-Labs, Oregon, USA) to generate identical arrays 156 in a 2×7 subarray format using Super Marathon printer (Arrayjet, UK). ..

other:

Article Title: Capture of Tumor Cells on Anti-EpCAM-Functionalized Poly(acrylic acid)-Coated Surfaces.
Article Snippet: Subscriber access provided by UNIV OF NEBRASKA - LINCOLN ACS Applied Materials & Interfaces is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

Article Title: Enzymatic Synthesis of High‐Density RNA Microarrays
Article Snippet: Final washing buffer (FWB; 0.1× SSC) RNase‐free water (Carl Roth, art.

Article Title: Alterations in the Glycan Composition of Serum Glycoproteins in Attention-Deficit Hyperactivity Disorder
Article Snippet: Then, appropriate multi-well microarray slide masks (Grace Bio-Labs, Bend, OR, USA) were applied on the slides, and a solution of 3% BSA in PBS was applied onto the unreacted epoxy groups on the slide surfaces for 1 h at room temperature.

Article Title: Alterations in the Glycan Composition of Serum Glycoproteins in Attention-Deficit Hyperactivity Disorder.
Article Snippet: Then, appropriate multi-well microarray slide masks (Grace Bio-Labs, Bend, OR, USA) were applied on the slides, and a solution of 3% BSA in PBS was applied onto the unreacted epoxy groups on the slide surfaces for 1 h at room temperature.

cDNA Synthesis:

Article Title: Triple-serotype chimeric oncolytic adenovirus exerts multiple synergistic mechanisms against solid tumors
Article Snippet: .. On-slide tissue permeabilization, cDNA synthesis and sequencing: After brightfield imaging, the ST slide was prewarmed to 42 °C and attached to a microarray slide module (Grace Biolabs). i. Permeabilization Enzyme (70 μl) was added to B1 along the side of the well (30 min) and tap cassette; ii. ..

Sequencing:

Article Title: Triple-serotype chimeric oncolytic adenovirus exerts multiple synergistic mechanisms against solid tumors
Article Snippet: .. On-slide tissue permeabilization, cDNA synthesis and sequencing: After brightfield imaging, the ST slide was prewarmed to 42 °C and attached to a microarray slide module (Grace Biolabs). i. Permeabilization Enzyme (70 μl) was added to B1 along the side of the well (30 min) and tap cassette; ii. ..

Imaging:

Article Title: Triple-serotype chimeric oncolytic adenovirus exerts multiple synergistic mechanisms against solid tumors
Article Snippet: .. On-slide tissue permeabilization, cDNA synthesis and sequencing: After brightfield imaging, the ST slide was prewarmed to 42 °C and attached to a microarray slide module (Grace Biolabs). i. Permeabilization Enzyme (70 μl) was added to B1 along the side of the well (30 min) and tap cassette; ii. ..

Microscopy:

Article Title: A Novel Mass Spectrometry Platform for Multiplexed N-glycoprotein Biomarker Discovery from Patient Biofluids by Antibody Panel Based N-glycan Imaging
Article Snippet: Slides were imaged on a solariX Legacy 7T FT-ICR mass spectrometer (Bruker) equipped with a matrix-assisted laser desorption/ionization (MALDI) source. .. Nitrocellulose-coated glass microscope slides (PATH microarray slides) and well slide modules (ProPlate Multi-Array Slide System, 24-well) were obtained from Grace Bio-Labs (Bend, OR). .. Trifluoroacetic acid, α-cyano-4-hydroxycinnamic acid, octyl-β-D-glucopyranoside, human alpha-1-antitrypsin, and stock human serum were obtained from Sigma Aldrich (St. Louis, MO).

Control:

Article Title: SARS-CoV-2 Antibody Signatures for Predicting the Outcome of COVID-19
Article Snippet: .. 150 151 Protein microarray fabrication 152 The microarray used for serum IgM and IgG profiling was prepared as described previously.17, 23 20 153 proteins of SARS-CoV-2 with indicated concentrations, along with the negative (GST, Biotin-154 control, and eGFP) and positive controls (Human IgG, Human IgM, and ACE2-Fc), were printed in 155 quadruplicate on PATH substrate slide (Grace Bio-Labs, Oregon, USA) to generate identical arrays 156 in a 2×7 subarray format using Super Marathon printer (Arrayjet, UK). ..



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Mean <t>microarray</t> antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.
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Mean <t>microarray</t> antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.
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Image Search Results


Mean microarray antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.

Journal: Journal of Clinical Microbiology

Article Title: Proteome microarray-guided identification of mycobacterial antigens and ELISA-based peptide mapping for improved serological detection of Mycobacterium bovis infection in European badgers

doi: 10.1128/jcm.01260-25

Figure Lengend Snippet: Mean microarray antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.

Article Snippet: Translated proteins and IVTT controls were printed onto custom nitrocellulose-coated microarray slides (Grace Bio-Labs, Bend, OR, USA) using a GeneMachines OmniGrid Accent Microarray Printer.

Techniques: Microarray, Infection, Comparison

Mean microarray antigen spot intensities from TB-infected and BCG-vaccinated badger serum samples. Gray bars represent sera from TB-infected badgers collected 12–13 weeks after experimental infection; white bars represent sera from badgers that received ( a ) a single BCG vaccination or ( b ) two BCG vaccinations. Mean spot intensities (log₂-normalized) are shown with 95% confidence interval error bars. Independent-samples t -tests were used to compare antigen intensities between TB-infected and BCG-vaccinated groups. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown; ***: P < 0.001.

Journal: Journal of Clinical Microbiology

Article Title: Proteome microarray-guided identification of mycobacterial antigens and ELISA-based peptide mapping for improved serological detection of Mycobacterium bovis infection in European badgers

doi: 10.1128/jcm.01260-25

Figure Lengend Snippet: Mean microarray antigen spot intensities from TB-infected and BCG-vaccinated badger serum samples. Gray bars represent sera from TB-infected badgers collected 12–13 weeks after experimental infection; white bars represent sera from badgers that received ( a ) a single BCG vaccination or ( b ) two BCG vaccinations. Mean spot intensities (log₂-normalized) are shown with 95% confidence interval error bars. Independent-samples t -tests were used to compare antigen intensities between TB-infected and BCG-vaccinated groups. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown; ***: P < 0.001.

Article Snippet: Translated proteins and IVTT controls were printed onto custom nitrocellulose-coated microarray slides (Grace Bio-Labs, Bend, OR, USA) using a GeneMachines OmniGrid Accent Microarray Printer.

Techniques: Microarray, Infection, Comparison